MAP1LC3B (phospho T12) polyclonal antibody
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More Files
- More Functions
Dot Blot (Peptide)
Dot blot analysis of MAP1LC3B (phospho T12) polyclonal antibody (Cat # PAB8153) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP1LC3B.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T12 of human MAP1LC3B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of MAP1LC3B (phospho T12) polyclonal antibody (Cat # PAB8153) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — MAP1LC3B
Entrez GeneID
81631Protein Accession#
NP_073729;Q9GZQ8Gene Name
MAP1LC3B
Gene Alias
LC3B, MAP1A/1BLC3
Gene Description
microtubule-associated protein 1 light chain 3 beta
Gene Ontology
HyperlinkGene Summary
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq
Other Designations
microtubule-associated proteins 1A/1B light chain 3
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Interactome
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Publication Reference
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Autophagy in metazoans: cell survival in the land of plenty.
Lum JJ, DeBerardinis RJ, Thompson CB.
Nature Reviews. Molecular Cell Biology 2005 Jun; 6(6):439.
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Autophagy: dual roles in life and death?
Baehrecke EH.
Nature Reviews. Molecular Cell Biology 2005 Jun; 6(6):505.
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Degrade or die: a dual function for autophagy in the plant immune response.
Greenberg JT.
Developmental Cell 2005 Jun; 8(6):799.
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Autophagy in metazoans: cell survival in the land of plenty.
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