MAP1LC3B monoclonal antibody, clone RM293
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) HeLa cells untreated with chloroquine (CHQ), (2) HeLa cells treated with chloroquine (CHQ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain.
Immunocytochemistry
Immunocytochemistry staining of Hela cells untreated or treated with chloroquine (CHQ) (red). Actin filaments labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MAP1LC3B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to N-terminus of human MAP1LC3B.
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts to human LC3B (Autophagy-related protein LC3 B) (Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:100-200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-200)
Western Blot (1:100-200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) HeLa cells untreated with chloroquine (CHQ), (2) HeLa cells treated with chloroquine (CHQ).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain.Immunocytochemistry
Immunocytochemistry staining of Hela cells untreated or treated with chloroquine (CHQ) (red). Actin filaments labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue). -
Gene Info — MAP1LC3B
Entrez GeneID
81631Gene Name
MAP1LC3B
Gene Alias
LC3B, MAP1A/1BLC3
Gene Description
microtubule-associated protein 1 light chain 3 beta
Gene Ontology
HyperlinkGene Summary
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq
Other Designations
microtubule-associated proteins 1A/1B light chain 3
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Interactome
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