MAP1LC3B polyclonal antibody

Catalog # PAB29556

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Price

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Quantity

Size:200 uL
Price: USD $ 329.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western blot analysis of Lane 1: Nontransfected 293 cell lysates (2 ug/lane), Lane 2: Transiently transfected 293 cell lysates (2 ug/lane) with MAP1LC3B polyclonal antibody (Cat # PAB29556) at 1:1000 dilution.

Immunofluorescence
Application

Immunofluorescence

Immunofluorescent staining of Chloroquine (50 uM, 16h) treated (A) and untreated (B) U251 cells with MAP1LC3B polyclonal antibody (Cat # PAB29556) at 1:100 dilution.

  • Specification

    Product Description

    Rabbit polyclonal antibody raised against partial synthetic protein of human MAP1LC3B.

    Immunogen

    A synthetic peptide (conjugated with KLH) corresponding to amino acids 1-30 at N-terminus of human MAP1LC3B.

    Host

    Rabbit

    Theoretical MW (kDa)

    14.7

    Reactivity

    Human, Rat

    Form

    Liquid

    Purification

    Ammonium sulfate precipitation

    Isotype

    Ig

    Recommend Usage

    Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Transfected lysate)

    Western blot analysis of Lane 1: Nontransfected 293 cell lysates (2 ug/lane), Lane 2: Transiently transfected 293 cell lysates (2 ug/lane) with MAP1LC3B polyclonal antibody (Cat # PAB29556) at 1:1000 dilution.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunofluorescence

    Immunofluorescent staining of Chloroquine (50 uM, 16h) treated (A) and untreated (B) U251 cells with MAP1LC3B polyclonal antibody (Cat # PAB29556) at 1:100 dilution.
  • Gene Info — MAP1LC3B

    Entrez GeneID

    81631

    Gene Name

    MAP1LC3B

    Gene Alias

    LC3B, MAP1A/1BLC3

    Gene Description

    microtubule-associated protein 1 light chain 3 beta

    Gene Ontology

    Hyperlink

    Gene Summary

    The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq

    Other Designations

    microtubule-associated proteins 1A/1B light chain 3

  • Interactome
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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