Immunoblots of Phospho-MAP1LC3A in CHO cell culture. MAP1LC3 and MAP1LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066). MAP1LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of MAP1LC3 with alanine. WT = wildtype MAP1LC3-transfected cell lysates; S12A = MAP1LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no MAP1LC3 gene. Molecular size: MAP1LC3-I = 16 KDa, and MAP1LC3-II = 14 KDa.
Dot Blot (Peptide)
Dot blot analysis of MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066) and Nonphospho-MAP1LC3 antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq