MAP1LC3A (Cleaved) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysates with MAP1LC3A (Cleaved) polyclonal antibody (Cat # PAB29557) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart tissue with MAP1LC3A (Cleaved) polyclonal antibody (Cat # PAB29557) at 1:10-50 dilution.
Immunocytochemistry
Immunocytochemical anyalysis of NIH/3T3 cells with MAP1LC3A (Cleaved) polyclonal antibody (Cat # PAB29557) at 1:10-50 dilution.
Immunofluorescence
Immunofluorescent staining of Chloroquine (50 uM, 16h) treated (A) and untreated (B) HeLa cells with MAP1LC3A (Cleaved) polyclonal antibody (Cat # PAB29557) shows vesicles staining at 1: 25 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial synthetic protein of human cleaved MAP1LC3A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 89-120 of human cleaved MAP1LC3A.
Host
Rabbit
Theoretical MW (kDa)
14.3
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A purification
Isotype
Ig
Recommend Usage
Immunocytochemistry (1:10-50)
Immunofluorescence (1:25)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysates with MAP1LC3A (Cleaved) polyclonal antibody (Cat # PAB29557) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart tissue with MAP1LC3A (Cleaved) polyclonal antibody (Cat # PAB29557) at 1:10-50 dilution.Immunocytochemistry
Immunocytochemical anyalysis of NIH/3T3 cells with MAP1LC3A (Cleaved) polyclonal antibody (Cat # PAB29557) at 1:10-50 dilution.Immunofluorescence
Immunofluorescent staining of Chloroquine (50 uM, 16h) treated (A) and untreated (B) HeLa cells with MAP1LC3A (Cleaved) polyclonal antibody (Cat # PAB29557) shows vesicles staining at 1: 25 dilution. -
Gene Info — MAP1LC3A
Entrez GeneID
84557Gene Name
MAP1LC3A
Gene Alias
LC3, LC3A, MAP1ALC3, MAP1BLC3
Gene Description
microtubule-associated protein 1 light chain 3 alpha
Omim ID
601242Gene Ontology
HyperlinkGene Summary
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
MAP1 light chain 3-like protein 1|MAP1A/1B light chain 3 A|MAP1A/MAP1B LC3 A|OTTHUMP00000030696|OTTHUMP00000030697|OTTHUMP00000030698|microtubule-associated proteins 1A/1B light chain 3
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