MAP1LC3A recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1:SHG-44 whole cell lysate Lane2:The brain tissue lysate of Mouse Lane3:The liver tissue lysate of Mouse Lane4:The skeletal muscle lysate of Mouse with MAP1LC3A recombinant monoclonal antibody (Cat # RAB02751) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver tissue using MAP1LC3A recombinant monoclonal antibody (Cat # RAB02751). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using MAP1LC3A recombinant monoclonal antibody (Cat # RAB02751)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against MAP1LC3A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant MAP1LC3A.
Theoretical MW (kDa)
14
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of MAP1LC3A and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:SHG-44 whole cell lysate Lane2:The brain tissue lysate of Mouse Lane3:The liver tissue lysate of Mouse Lane4:The skeletal muscle lysate of Mouse with MAP1LC3A recombinant monoclonal antibody (Cat # RAB02751) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver tissue using MAP1LC3A recombinant monoclonal antibody (Cat # RAB02751). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using MAP1LC3A recombinant monoclonal antibody (Cat # RAB02751)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — MAP1LC3A
Entrez GeneID
84557Protein Accession#
Q9H492Gene Name
MAP1LC3A
Gene Alias
LC3, LC3A, MAP1ALC3, MAP1BLC3
Gene Description
microtubule-associated protein 1 light chain 3 alpha
Omim ID
601242Gene Ontology
HyperlinkGene Summary
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
MAP1 light chain 3-like protein 1|MAP1A/1B light chain 3 A|MAP1A/MAP1B LC3 A|OTTHUMP00000030696|OTTHUMP00000030697|OTTHUMP00000030698|microtubule-associated proteins 1A/1B light chain 3
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Interactome
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