HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human MAP3K7 with DYKDDDDK tag probed with MAP3K7 polyclonal antibody (Cat # PAB7038) (1 ug/mL) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with MAP3K7 polyclonal antibody (Cat # PAB7038) (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq