DDX5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
DDX5 polyclonal antibody (Cat # PAB6597) staining (0.5 ug/mL) of A-431 cell lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DDX5 polyclonal antibody (Cat # PAB6597, 2.5 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DDX5.
Immunogen
A synthetic peptide corresponding to C-terminus of human DDX5.
Sequence
C-PMIGYPMPTGYSQ
Host
Goat
Theoretical MW (kDa)
69
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western blot (0.11-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-5 ug/mL)
Immunofluorescence (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
DDX5 polyclonal antibody (Cat # PAB6597) staining (0.5 ug/mL) of A-431 cell lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DDX5 polyclonal antibody (Cat # PAB6597, 2.5 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — DDX5
Entrez GeneID
1655Protein Accession#
NP_004387.1Gene Name
DDX5
Gene Alias
DKFZp434E109, DKFZp686J01190, G17P1, HLR1, HUMP68, p68
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Omim ID
180630Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX5|DEAD box-5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)
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Interactome
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Disease
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Publication Reference
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Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction.
Iggo R, Gough A, Xu W, Lane DP, Spurr NK.
PNAS 1989 Aug; 86(16):6211.
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Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction.
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