Goat polyclonal antibody raised against synthetic peptide of DDX5.
A synthetic peptide corresponding to C-terminus of human DDX5.
Theoretical MW (kDa):
Antigen affinity purification
ELISA (1:64000) Western blot (0.11-0.3 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-5 ug/mL) Immunofluorescence (1:100) The optimal working dilution should be determined by the end user.
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
DDX5 polyclonal antibody (Cat # PAB6597) staining (0.5 ug/mL) of A-431 cell lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq