DDX5 recombinant monoclonal antibody, clone R04-2K2
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: C6, Lane 2: 3T3 and Lane 3: Hela lysates with DDX5 recombinant monoclonal antibody, clone R04-2K2 (Cat # RAB02326).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human DDX5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human DDX5.
Theoretical MW (kDa)
Calculated MW: 69 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: C6, Lane 2: 3T3 and Lane 3: Hela lysates with DDX5 recombinant monoclonal antibody, clone R04-2K2 (Cat # RAB02326).Immunohistochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — DDX5
Entrez GeneID
1655Protein Accession#
P17844Gene Name
DDX5
Gene Alias
DKFZp434E109, DKFZp686J01190, G17P1, HLR1, HUMP68, p68
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Omim ID
180630Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX5|DEAD box-5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)
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Interactome
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Disease
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