DDX5 recombinant monoclonal antibody, clone 2C3
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: HepG2 whole cell lysate; Lane 2: HT-29 whole cell lysate; Lane 3: 293T whole cell lysate; Lane 4: Jurkat whole cell lysate; Lane 5: Mouse brain tissue.
Immunohistochemistry
Immunohistochemistry image of DDX5 recombinant monoclonal antibody, clone 2C3 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of HepG2 Cells with DDX5 recombinant monoclonal antibody, clone 2C3 at 1:50, counter-stained with DAPI.
Flow Cytometry
Overlay histogram showing Hela cells stained with DDX5 recombinant monoclonal antibody, clone 2C3 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse DDX5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human DDX5.
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HepG2 whole cell lysate; Lane 2: HT-29 whole cell lysate; Lane 3: 293T whole cell lysate; Lane 4: Jurkat whole cell lysate; Lane 5: Mouse brain tissue.Immunohistochemistry
Immunohistochemistry image of DDX5 recombinant monoclonal antibody, clone 2C3 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of HepG2 Cells with DDX5 recombinant monoclonal antibody, clone 2C3 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing Hela cells stained with DDX5 recombinant monoclonal antibody, clone 2C3 (red line) at 1:50. -
Gene Info — DDX5
Entrez GeneID
1655Protein Accession#
P17844Gene Name
DDX5
Gene Alias
DKFZp434E109, DKFZp686J01190, G17P1, HLR1, HUMP68, p68
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Omim ID
180630Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX5|DEAD box-5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)
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Interactome
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Disease
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