NFKBIA polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NFKBIA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NFKBIA.
Host
Rabbit
Reactivity
Human
Specificity
This antibody recognize synthetic peptide corresponding to sumoylation site of human NFKBIA.
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The NFKBIA polyclonal antibody (Cat # PAB4345) is used in Western blot to detect NFKBIA in Jurkat cell lysate .Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFKBIA
Entrez GeneID
4792Protein Accession#
NP_065390Gene Name
NFKBIA
Gene Alias
IKBA, MAD-3, NFKBI
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Omim ID
164008Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
IkappaBalpha|nuclear factor of kappa light chain gene enhancer in B-cells
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Interactomes
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Pathways
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Diseases
- Adenocarcinoma
- Alcoholism
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
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Publication Reference
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Okadaic acid induces sustained activation of NFkappaB and degradation of the nuclear IkappaBalpha in human neutrophils.
Miskolci V, Castro-Alcaraz S, Nguyen P, Vancura A, Davidson D, Vancurova I.
Archives of Biochemistry and Biophysics 2003 Sep; 417(1):44.
Application:WB-Ce, Human, Neutrophils.
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HSP27 is a ubiquitin-binding protein involved in I-kappaBalpha proteasomal degradation.
Parcellier A, Schmitt E, Gurbuxani S, Seigneurin-Berny D, Pance A, Chantome A, Plenchette S, Khochbin S, Solary E, Garrido C.
Molecular and Cellular Biology 2003 Aug; 23(16):5790.
Application:WB-Tr, Human, U937 cells.
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Phosphorylation of threonine 10 on CKBBP1/SAG/ROC2/Rbx2 by protein kinase CKII promotes the degradation of IkappaBalpha and p27Kip1.
Kim YS, Lee JY, Son MY, Park W, Bae YS.
The Journal of Biological Chemistry 2003 Aug; 278(31):28462.
Application:WB-Tr, Human, HeLa cells.
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Okadaic acid induces sustained activation of NFkappaB and degradation of the nuclear IkappaBalpha in human neutrophils.
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