NFKBIA (phospho S32) recombinant monoclonal antibody, clone 2D6
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: A549 whole cell lysate (not treated) with NFKBIA (phospho S32) recombinant monoclonal antibody, clone 2D6 (Cat # RAB04243).
Immunofluorescence
Immunofluorescent staining of A549 cells with NFKBIA (phospho S32) recombinant monoclonal antibody, clone 2D6 (Cat # RAB04243) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NFKBIA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S32 of human NFKBIA.
Theoretical MW (kDa)
Calculated MW: 39 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: A549 whole cell lysate (not treated) with NFKBIA (phospho S32) recombinant monoclonal antibody, clone 2D6 (Cat # RAB04243).Immunofluorescence
Immunofluorescent staining of A549 cells with NFKBIA (phospho S32) recombinant monoclonal antibody, clone 2D6 (Cat # RAB04243) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — NFKBIA
Entrez GeneID
4792Protein Accession#
P25963Gene Name
NFKBIA
Gene Alias
IKBA, MAD-3, NFKBI
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Omim ID
164008Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
IkappaBalpha|nuclear factor of kappa light chain gene enhancer in B-cells
-
Interactome
-
Pathway
-
Disease
- Adenocarcinoma
- Alcoholism
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
+ View More Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com