NFKBIA recombinant monoclonal antibody, clone 1E9
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: Jurkat whole cell lysate, Lane 4: Raji whole cell lysate, Lane 5: PC3 whole cell lysate and Lane 6: THP-1 whole cell lysate with NFKBIA recombinant monoclonal antibody, clone 1E9 (Cat # RAB04345).
Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with NFKBIA recombinant monoclonal antibody, clone 1E9 (Cat # RAB04345) (diluated at 1:79.75).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with NFKBIA recombinant monoclonal antibody, clone 1E9 (Cat # RAB04345).
Lane 1: rabbit control IgG, Lane 2: RAB04345 precipitates and Lane 3: Input (Hela whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NFKBIA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NFKBIA.
Theoretical MW (kDa)
Calculated MW: 36 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: Jurkat whole cell lysate, Lane 4: Raji whole cell lysate, Lane 5: PC3 whole cell lysate and Lane 6: THP-1 whole cell lysate with NFKBIA recombinant monoclonal antibody, clone 1E9 (Cat # RAB04345).Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with NFKBIA recombinant monoclonal antibody, clone 1E9 (Cat # RAB04345) (diluated at 1:79.75).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with NFKBIA recombinant monoclonal antibody, clone 1E9 (Cat # RAB04345).
Lane 1: rabbit control IgG, Lane 2: RAB04345 precipitates and Lane 3: Input (Hela whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFKBIA
Entrez GeneID
4792Protein Accession#
P25963Gene Name
NFKBIA
Gene Alias
IKBA, MAD-3, NFKBI
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Omim ID
164008Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
IkappaBalpha|nuclear factor of kappa light chain gene enhancer in B-cells
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Interactome
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Pathway
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Disease
- Adenocarcinoma
- Alcoholism
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
+ View More Disease
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