Western blot analysis of NRAS polyclonal antibody (Cat # PAB3461) in CEM cell line lysates (35 ug/lane). NRAS (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of NRAS (arrow) using rabbit NRAS polyclonal antibody (Cat # PAB3461). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NRAS gene (Lane 2) (Origene Technologies).
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq
N-ras protein part 4,OTTHUMP00000013879,v-ras neuroblastoma RAS viral oncogene homolog