NRAS recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1:MCF7 whole cell lysate Lane2:293T whole cell lysate Lane3:The brain tissue lysate of Mouse with NRAS recombinant monoclonal antibody (Cat # RAB02661) at 1:1000 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells with NRAS recombinant monoclonal antibody (Cat # RAB02661) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against NRAS.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant NRAS.
Theoretical MW (kDa)
18
Reactivity
Human, Mouse, Rat, Zebra fish
Specificity
This antibody is predicted to react with H-Ras, N-Ras and K-Ras.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:10-1:100)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:MCF7 whole cell lysate Lane2:293T whole cell lysate Lane3:The brain tissue lysate of Mouse with NRAS recombinant monoclonal antibody (Cat # RAB02661) at 1:1000 dilution.Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of Hela cells with NRAS recombinant monoclonal antibody (Cat # RAB02661) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. -
Gene Info — NRAS
Entrez GeneID
4893Protein Accession#
P01111Gene Name
NRAS
Gene Alias
ALPS4, N-ras, NRAS1
Gene Description
neuroblastoma RAS viral (v-ras) oncogene homolog
Gene Ontology
HyperlinkGene Summary
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq
Other Designations
N-ras protein part 4|OTTHUMP00000013879|v-ras neuroblastoma RAS viral oncogene homolog
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Interactome
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Pathway
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Disease
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