NRAS recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Hela (A), CHOK1 (B), C6 (C), NIH3T3 (D), mouse brain (E) whole cell lysates with NRAS recombinant monoclonal antibody (Cat # RAB02606).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NRAS.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human NRAS.
Theoretical MW (kDa)
21
Reactivity
Hamster, Human, Mouse, Rat
Specificity
Recognizes endogenous levels of NRAS protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Hela (A), CHOK1 (B), C6 (C), NIH3T3 (D), mouse brain (E) whole cell lysates with NRAS recombinant monoclonal antibody (Cat # RAB02606).Immunoprecipitation
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Gene Info — NRAS
Entrez GeneID
4893Protein Accession#
P01111Gene Name
NRAS
Gene Alias
ALPS4, N-ras, NRAS1
Gene Description
neuroblastoma RAS viral (v-ras) oncogene homolog
Gene Ontology
HyperlinkGene Summary
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq
Other Designations
N-ras protein part 4|OTTHUMP00000013879|v-ras neuroblastoma RAS viral oncogene homolog
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Interactome
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Pathway
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Disease
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