PIK3R2 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIK3R2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PIK3R2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PIK3R2 polyclonal antibody (Cat # PAB3222) in A-549 cell lysate. PIK3R2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIK3R2 polyclonal antibody (Cat # PAB3222) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PIK3R2
Entrez GeneID
5296Protein Accession#
O00459Gene Name
PIK3R2
Gene Alias
P85B, p85, p85-BETA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Omim ID
603157Gene Ontology
HyperlinkGene Summary
O
Other Designations
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)|phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)|phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
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Interactomes
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Pathways
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Publication Reference
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An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme.
Janssen JW, Schleithoff L, Bartram CR, Schulz AS.
Oncogene 1998 Apr; 16(13):1767.
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Chromosomal localization of human p85 alpha, a subunit of phosphatidylinositol 3-kinase, and its homologue p85 beta.
Volinia S, Patracchini P, Otsu M, Hiles I, Gout I, Calzolari E, Bernardi F, Rooke L, Waterfield MD.
Oncogene 1992 Apr; 7(4).
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Purification and characterization of phosphoinositide 3-kinase from rat liver.
Carpenter CL, Duckworth BC, Auger KR, Cohen B, Schaffhausen BS, Cantley LC.
The Journal of Biological Chemistry 1990 Nov; 265(32):19704.
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An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme.
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