PIK3R2 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Raji whole cell lysate Lane2:Hela whole cell lysate Lane3:MCF7 whole cell lysate Lane4:U937 whole cell lysate with PIK3R2 recombinant monoclonal antibody (Cat # RAB02754) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded rat testis tissue using PIK3R2 recombinant monoclonal antibody (Cat # RAB02754). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using PIK3R2 recombinant monoclonal antibody (Cat # RAB02754)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against PIK3R2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant PIK3R2.
Theoretical MW (kDa)
85
Reactivity
Human, Rat
Specificity
This antibody detects endogenous levels of PI3K p85beta and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Raji whole cell lysate Lane2:Hela whole cell lysate Lane3:MCF7 whole cell lysate Lane4:U937 whole cell lysate with PIK3R2 recombinant monoclonal antibody (Cat # RAB02754) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded rat testis tissue using PIK3R2 recombinant monoclonal antibody (Cat # RAB02754). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using PIK3R2 recombinant monoclonal antibody (Cat # RAB02754)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — PIK3R2
Entrez GeneID
5296Protein Accession#
O00459Gene Name
PIK3R2
Gene Alias
P85B, p85, p85-BETA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Omim ID
603157Gene Ontology
HyperlinkGene Summary
O
Other Designations
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)|phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)|phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
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Interactome
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Pathway
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