SMAD3 (phospho S204) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) Huvec cell lysate, (2) A-549 cell lysate, and (3) 3T3 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain (left). Negative control was pre-absorbed by immunogen peptide (right).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human SMAD3 (phospho S204).
Immunogen
A synthetic peptide corresponding to amino acids 140-220 of human SMAD3 (phospho S204).
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Smad3 protein only when phosphorylated at S204.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
ELISA (1:20000)
Immunohistochemistry (1:100-300)
Immunofluorescence (1:200-1000)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) Huvec cell lysate, (2) A-549 cell lysate, and (3) 3T3 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain (left). Negative control was pre-absorbed by immunogen peptide (right).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMAD3
Entrez GeneID
4088Protein Accession#
P84022Gene Name
SMAD3
Gene Alias
DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396
Gene Description
SMAD family member 3
Omim ID
603109Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|mad homolog JV15-2|mad protein homolog|mothers against decapentaplegic homolog 3
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Interactome
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Pathway
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Disease
- Alzheimer disease
- Anemia
- Asthma
- Bacteremia
- Breast cancer
- Breast Neoplasms
- Cleft Lip
- Cleft Palate
- Coronary Artery Disease
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