SMAD3 (phospho S423/S425) recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against SMAD3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant SMAD3.
Theoretical MW (kDa)
55
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of Smad3 only when phosphorylated at Ser423 and/or Ser425.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:A431 whole cell lysate Lane2:A431 whole cell lysate treated with TGF-beta with SMAD3 (phospho S423/S425) recombinant monoclonal antibody (Cat # RAB02760) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using SMAD3 (phospho S423/S425) recombinant monoclonal antibody (Cat # RAB02760). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of A431 cells using SMAD3 (phospho S423/S425) recombinant monoclonal antibody (Cat # RAB02760)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — SMAD3
Entrez GeneID
4088Protein Accession#
P84022Gene Name
SMAD3
Gene Alias
DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396
Gene Description
SMAD family member 3
Omim ID
603109Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|mad homolog JV15-2|mad protein homolog|mothers against decapentaplegic homolog 3
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Interactome
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Pathway
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Disease
- Alzheimer disease
- Anemia
- Asthma
- Bacteremia
- Breast cancer
- Breast Neoplasms
- Cleft Lip
- Cleft Palate
- Coronary Artery Disease
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