SMAD3 recombinant monoclonal antibody, clone R02-1I2
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1: 3T3 and Lane2: C6 lysates with SMAD3 recombinant monoclonal antibody, clone R02-1I2 (Cat # RAB01907).
Western Blot
Western blot analysis of Jurkat lysates with SMAD3 recombinant monoclonal antibody, clone R02-1I2 (Cat # RAB01907).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SMAD3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human SMAD3.
Theoretical MW (kDa)
Calculated MW: 48 kD
Reactivity
Hamster, Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1: 3T3 and Lane2: C6 lysates with SMAD3 recombinant monoclonal antibody, clone R02-1I2 (Cat # RAB01907).Western Blot
Western blot analysis of Jurkat lysates with SMAD3 recombinant monoclonal antibody, clone R02-1I2 (Cat # RAB01907).Immunoprecipitation
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Gene Info — SMAD3
Entrez GeneID
4088Protein Accession#
P84022Gene Name
SMAD3
Gene Alias
DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396
Gene Description
SMAD family member 3
Omim ID
603109Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|mad homolog JV15-2|mad protein homolog|mothers against decapentaplegic homolog 3
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Interactome
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Pathway
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Disease
- Alzheimer disease
- Anemia
- Asthma
- Bacteremia
- Breast cancer
- Breast Neoplasms
- Cleft Lip
- Cleft Palate
- Coronary Artery Disease
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