MAPKAPK2 (phospho S272) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated RAW264.7 cells, Lane 2: antigen-specific peptide treated RAW264.7 cells with MAPKAPK2 (phospho S272) polyclonal antibody (Cat# PAB29630) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by MAPKAPK2 (phospho S272) polyclonal antibody (Cat# PAB29630) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MAPKAPK2.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S272 of human MAPKAPK2.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human, Mouse, Rabbit
Specificity
MAPKAPK2 (phospho S272) polyclonal antibody detects endogenous level of MAPKAPK2 only when phosphorylated at serine 272.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated RAW264.7 cells, Lane 2: antigen-specific peptide treated RAW264.7 cells with MAPKAPK2 (phospho S272) polyclonal antibody (Cat# PAB29630) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by MAPKAPK2 (phospho S272) polyclonal antibody (Cat# PAB29630) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — MAPKAPK2
Entrez GeneID
9261Protein Accession#
P49137Gene Name
MAPKAPK2
Gene Alias
MK2
Gene Description
mitogen-activated protein kinase-activated protein kinase 2
Omim ID
602006Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000034531|OTTHUMP00000034532
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Interactome
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Pathway
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