Products

Western Blot (Cell lysate)

Western blot analysis of Hela whole cell lysate, HEK293 whole cell lysate, THP-1 whole cell lysate with MAPKAPK2 recombinant monoclonal antibody, clone 4H8 (Cat # RAB07445).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human breast cancer using MAPKAPK2 recombinant monoclonal antibody, clone 4H8 (Cat # RAB07445) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

9261

Protein Accession#

P49137

Gene Name

MAPKAPK2

Gene Alias

MK2

Gene Description

mitogen-activated protein kinase-activated protein kinase 2

Omim ID

602006

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq

Other Designations

OTTHUMP00000034531|OTTHUMP00000034532