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NFKB2 (phospho S866) polyclonal antibody

  • Catalog # : PAB29599
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human NFKB2.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S866 of human NFKB2.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 120
  • Reactivity:
  • Human, Mouse, Rabbit
  • Specificity:
  • NFKB2 (phospho S866) polyclonal antibody detects endogenous level of NFKB2 only when phosphorylated at serine 866.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100-1:200)
    Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: ovary cancer cells, Lane 2: synthesized peptide treated ovary cancer cells with NFKB2 (phospho S866) polyclonal antibody (Cat# PAB29599) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human breast cancer tissue by NFKB2 (phospho S866) polyclonal antibody (Cat# PAB29599) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of methanol-fixed Hela cells using NFKB2 (phospho S866) polyclonal antibody (Cat# PAB29599) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4791
  • Gene Name:
  • NFKB2
  • Gene Alias:
  • LYT-10,LYT10
  • Gene Description:
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
  • Gene Summary:
  • NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000020371,OTTHUMP00000059136,nuclear factor of kappa light chain gene enhancer in B-cells 2,nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
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