NFKB2 (phospho S870) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MDA-MB-435 cells, Lane 2: TNF-α treated (20 ng/mL, 5 min) MDA-MB-435 cells with NFKB2 (phospho S870) polyclonal antibody (Cat# PAB29600) at 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue with NFKB2 (phospho S870) polyclonal antibody (Cat# PAB29600) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells using NFKB2 (phospho S870) polyclonal antibody (Cat# PAB29600) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human NFKB2.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S870 of human NFKB2.
Host
Rabbit
Theoretical MW (kDa)
120
Reactivity
Human, Mouse, Rabbit
Specificity
NFKB2 (phospho S870) polyclonal antibody detects endogenous level of NFKB2 only when phosphorylated at serine 870.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MDA-MB-435 cells, Lane 2: TNF-α treated (20 ng/mL, 5 min) MDA-MB-435 cells with NFKB2 (phospho S870) polyclonal antibody (Cat# PAB29600) at 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue with NFKB2 (phospho S870) polyclonal antibody (Cat# PAB29600) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells using NFKB2 (phospho S870) polyclonal antibody (Cat# PAB29600) at 1:100-1:200 dilution. -
Gene Info — NFKB2
Entrez GeneID
4791Protein Accession#
Q00653Gene Name
NFKB2
Gene Alias
LYT-10, LYT10
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Omim ID
164012Gene Ontology
HyperlinkGene Summary
NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM
Other Designations
OTTHUMP00000020371|OTTHUMP00000059136|nuclear factor of kappa light chain gene enhancer in B-cells 2|nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
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Interactome
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Pathway
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Disease
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