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RPS6KA1 (phospho T573) polyclonal antibody

  • Catalog # : PAB29263
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RPS6KA1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T573 of human RPS6KA1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 95
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • RPS6KA1 (phospho T573) polyclonal antibody detects endogenous levels of human RPS6KA1 only when phosphorylated at threonine 573.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: UV treated 293 cells, Lane 2: antigen-specific peptide treated 293 cells with RPS6KA1 (phospho T573) polyclonal antibody (Cat# PAB29263) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with RPS6KA1 (phospho T573) polyclonal antibody (Cat# PAB29263) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6195
  • Gene Name:
  • RPS6KA1
  • Gene Alias:
  • HU-1,MAPKAPK1A,RSK,RSK1
  • Gene Description:
  • ribosomal protein S6 kinase, 90kDa, polypeptide 1
  • Gene Summary:
  • This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000004113,S6K-alpha 1,dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1),p90-RSK 1,ribosomal protein S6 kinase alpha 1,ribosomal protein S6 kinase, 90kD, 1,ribosomal protein S6 kinase, 90kD, polypeptide 1
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