ZAP70 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
ZAP70 polyclonal antibody (Cat # PAB24606) (0.3 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ZAP70.
Immunogen
A synthetic peptide corresponding to internal region of human ZAP70.
Sequence
C-SDGYTPEPARITSPD
Host
Goat
Theoretical MW (kDa)
70
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_001070.2) only.
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ZAP70 polyclonal antibody (Cat # PAB24606) (0.3 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ZAP70
Entrez GeneID
7535Protein Accession#
NP_001070.2Gene Name
ZAP70
Gene Alias
FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70
Gene Description
zeta-chain (TCR) associated protein kinase 70kDa
Omim ID
176947Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq
Other Designations
syk-related tyrosine kinase|zeta-chain (TCR) associated protein kinase (70 kD)|zeta-chain associated protein kinase 70kDa|zeta-chain associated protein kinase, 70kD
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Interactome
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Pathway
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Disease
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