BAK1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BAK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human BAK1.
Host
Rabbit
Reactivity
Human
Specificity
BH3 Domain Specific
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The BAK1 polyclonal antibody (Cat # PAB2439) is used in Western blot to detect BAK1 in A-375 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the BAK1 polyclonal antibody (Cat # PAB2439) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — BAK1
Entrez GeneID
578Protein Accession#
NP_001179;Q16611Gene Name
BAK1
Gene Alias
BAK, BAK-LIKE, BCL2L7, CDN1, MGC117255, MGC3887
Gene Description
BCL2-antagonist/killer 1
Omim ID
600516Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq
Other Designations
BCL2-like 7 protein|Bcl-2 homologous antagonist/killer|OTTHUMP00000016211|OTTHUMP00000039620|apoptosis regulator BAK|pro-apoptotic protein BAK
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Interactome
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Disease
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Publication Reference
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Nonredundant role of Bax and Bak in Bid-mediated apoptosis.
Cartron PF, Juin P, Oliver L, Martin S, Meflah K, Vallette FM.
Molecular and Cellular Biology 2003 Jul; 23(13):4701.
Application:WB-Ce, Human, BeGBM, BdGBM cells.
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Association of Bax and Bak homo-oligomers in mitochondria. Bax requirement for Bak reorganization and cytochrome c release.
Mikhailov V, Mikhailova M, Degenhardt K, Venkatachalam MA, White E, Saikumar P.
The Journal of Biological Chemistry 2002 Nov; 278(7):5367.
Application:IF, Human, RPTC cells.
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Bcl-2 family member Bfl-1/A1 sequesters truncated bid to inhibit is collaboration with pro-apoptotic Bak or Bax.
Werner AB, de Vries E, Tait SW, Bontjer I, Borst J.
The Journal of Biological Chemistry 2002 Apr; 277(25):22781.
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Nonredundant role of Bax and Bak in Bid-mediated apoptosis.
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