BAK1 recombinant monoclonal antibody, clone 8D1
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: THP-1 whole cell lysate, Lane 4: 293 whole cell lysate, Lane 5: A549 whole cell lysate, Lane 6: K562 whole cell lysate and Lane 7: Colo320 whole cell lysate with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322).
Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human lymph node tissue with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322) (diluated at 1:90).
Immunoprecipitation
Immunoprecipitation analysis of HEK293 cell lysate with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322).
Lane 1: rabbit control IgG, Lane 2: RAB04322 precipitates and Lane 3: Input (293 whole cell lysate).
Flow Cytometry
Flow cytometric analysis of HeLa cells with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BAK1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human BAK1.
Theoretical MW (kDa)
Calculated MW: 24, 1
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: THP-1 whole cell lysate, Lane 4: 293 whole cell lysate, Lane 5: A549 whole cell lysate, Lane 6: K562 whole cell lysate and Lane 7: Colo320 whole cell lysate with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322).Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human lymph node tissue with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322) (diluated at 1:90).Immunoprecipitation
Immunoprecipitation analysis of HEK293 cell lysate with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322).
Lane 1: rabbit control IgG, Lane 2: RAB04322 precipitates and Lane 3: Input (293 whole cell lysate).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with BAK1 recombinant monoclonal antibody, clone 8D1 (Cat # RAB04322) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — BAK1
Entrez GeneID
578Protein Accession#
Q16611Gene Name
BAK1
Gene Alias
BAK, BAK-LIKE, BCL2L7, CDN1, MGC117255, MGC3887
Gene Description
BCL2-antagonist/killer 1
Omim ID
600516Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq
Other Designations
BCL2-like 7 protein|Bcl-2 homologous antagonist/killer|OTTHUMP00000016211|OTTHUMP00000039620|apoptosis regulator BAK|pro-apoptotic protein BAK
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Interactome
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Disease
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