LEFTY1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LEFTY1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 291-320 at C-terminus of human LEFTY1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LEFTY1 polyclonal antibody (Cat # PAB2064) in HL-60 cell line lysates (35 ug/lane). LEFTY1 (arrow) was detected using the purified Polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinomareacted with LEFTY1 polyclonal antibody (Cat # PAB2064), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — LEFTY1
Entrez GeneID
10637Protein Accession#
NP_066277;O75610Gene Name
LEFTY1
Gene Alias
LEFTB, LEFTYB
Gene Description
left-right determination factor 1
Omim ID
603037Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the TGF-beta family of proteins. A similar secreted protein in mouse plays a role in left-right asymmetry determination of organ systems during development. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq
Other Designations
OTTHUMP00000035572|left-right determination, factor B
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Pathway
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Disease
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Publication Reference
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Molecular analysis of LEFTY-expressing cells in early human embryoid bodies.
Dvash T, Sharon N, Yanuka O, Benvenisty N.
Stem Cells 2007 Feb; 25(2):465.
Application:IF, Human, Human embryonic stem cells.
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Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells requires activation of Smad2/3.
Besser D.
The Journal of Biological Chemistry 2004 Oct; 279(43):45076.
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Characterization and mutation analysis of human LEFTY A and LEFTY B, homologues of murine genes implicated in left-right axis development.
Kosaki K, Bassi MT, Kosaki R, Lewin M, Belmont J, Schauer G, Casey B.
American Journal of Human Genetics 1999 Mar; 64(3):712.
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Molecular analysis of LEFTY-expressing cells in early human embryoid bodies.
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