CTCFL polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with CTCFL polyclonal antibody (Cat # PAB20061) shows strong nuclear positivity in primary spermatocytes.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with CTCFL polyclonal antibody (Cat # PAB20061) at 1-4 ug/mL dilution shows positivity in nucleus, cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant CTCFL.
Immunogen
Recombinant protein corresponding to amino acids of human CTCFL.
Sequence
DGVCREKDHRSPSELEAQRTSGAFQDSVLEEEVELVLAPSEESEKYILTLQTVHFTSEAVELQDMSLLSIQQQEGVQVVVQQPGPGLLWLEEGPRQSLQQCVAISIQQELYSPQEMEVLQFHALEENVMVASEDSKLAVS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with CTCFL polyclonal antibody (Cat # PAB20061) shows strong nuclear positivity in primary spermatocytes.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with CTCFL polyclonal antibody (Cat # PAB20061) at 1-4 ug/mL dilution shows positivity in nucleus, cytoplasm. -
Gene Info — CTCFL
Entrez GeneID
140690Protein Accession#
Q8NI51Gene Name
CTCFL
Gene Alias
BORIS, CTCF-T, MGC163358, dJ579F20.2
Gene Description
CCCTC-binding factor (zinc finger protein)-like
Omim ID
607022Gene Ontology
HyperlinkGene Summary
CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. [provided by RefSeq
Other Designations
BORIS-like protein|CCCTC-binding factor-like protein|OTTHUMP00000174287|brother of the regulator of imprinted sites
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Interactome
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Disease
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