CTCFL polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot using CTCFL polyclonal antibody (Cat # PAB9983) shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead).
Lane 1 contains lysate from human prostate tissue.
Lane 2 contains lysate from human spleen tissue.
Apredominant band at ~75 KDa is observed.
Molecular weight estimation was made by comparison to prestained MW markers as indicated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CTCFL.
Immunogen
A synthetic peptide corresponding to amino acids 9-26 of human CTCFL.
Host
Rabbit
Reactivity
Chimpanzee, Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using CTCFL polyclonal antibody (Cat # PAB9983) shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead).
Lane 1 contains lysate from human prostate tissue.
Lane 2 contains lysate from human spleen tissue.
Apredominant band at ~75 KDa is observed.
Molecular weight estimation was made by comparison to prestained MW markers as indicated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CTCFL
Entrez GeneID
140690Protein Accession#
Q8NI51;AAM28645Gene Name
CTCFL
Gene Alias
BORIS, CTCF-T, MGC163358, dJ579F20.2
Gene Description
CCCTC-binding factor (zinc finger protein)-like
Omim ID
607022Gene Ontology
HyperlinkGene Summary
CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. [provided by RefSeq
Other Designations
BORIS-like protein|CCCTC-binding factor-like protein|OTTHUMP00000174287|brother of the regulator of imprinted sites
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Interactome
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Disease
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Publication Reference
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The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer.
Klenova EM, Morse HC 3rd, Ohlsson R, Lobanenkov VV.
Seminars in Cancer Biology 2002 Oct; 12(5):399.
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BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma.
Loukinov DI, Pugacheva E, Vatolin S, Pack SD, Moon H, Chernukhin I, Mannan P, Larsson E, Kanduri C, Vostrov AA, Cui H, Niemitz EL, Rasko JE, Docquier FM, Kistler M, Breen JJ, Zhuang Z, Quitschke WW, Renkawitz R, Klenova EM, Feinberg AP, Ohlsson R, Morse HC 3rd, Lobanenkov VV.
PNAS 2002 May; 99(10):6806.
Application：EMSA, IS, Yeast, Mouse, Pichia pastoris, Testis.
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The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer.
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