CARD10 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Jurkat (Lane 1) and MCF-7 (Lane 2) cell lysate with CARD10 polyclonal antibody (Cat # PAB18843) at 1 : 200 dilution.
Immunofluorescence
Immunofluorescent staining of HeLa cells showing cytoplasmic staining with CARD10 polyclonal antibody (Cat # PAB18843) at 1 : 100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CARD10.
Immunogen
A synthetic peptide corresponding to 15 amino acids at C-terminus of human CARD10.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:200-1:500)
Immunofluorescence (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.02% sodium azide
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat (Lane 1) and MCF-7 (Lane 2) cell lysate with CARD10 polyclonal antibody (Cat # PAB18843) at 1 : 200 dilution.Immunofluorescence
Immunofluorescent staining of HeLa cells showing cytoplasmic staining with CARD10 polyclonal antibody (Cat # PAB18843) at 1 : 100 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CARD10
Entrez GeneID
29775Protein Accession#
Q9BWT7Gene Name
CARD10
Gene Alias
BIMP1, CARMA3, MGC142219
Gene Description
caspase recruitment domain family, member 10
Omim ID
607209Gene Ontology
HyperlinkGene Summary
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM
Other Designations
Bcl10 binding protein and activator of NFKB|CARD-containing MAGUK 3 protein|caspase recruitment domain protein 10
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Interactome
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Disease
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