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Last updated: 2017/2/19
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EZR (phospho T353) polyclonal antibody

  • Catalog # : PAB18517
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of EZR.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T353 of human EZR.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is specific to EZR only when phosphorylated at T353.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol).
  • Storage Instruction:
  • Store at 4°C for three months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from A-431 cells, untreated or EGF-treated (200 ng/ml, 30 min), using EZR (phospho T353) polyclonal antibody (Cat # PAB18517).
    Peptide "+" means "peptide blocking".
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using EZR (phospho T353) polyclonal antibody (Cat # PAB18517).
    Peptide "+" means "peptide blocking".
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7430
  • Gene Name:
  • EZR
  • Gene Alias:
  • CVIL,CVL,DKFZp762H157,FLJ26216,MGC1584,VIL2
  • Gene Description:
  • ezrin
  • Gene Summary:
  • The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000017506,OTTHUMP00000017507,cytovillin 2,villin 2 (ezrin)
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