EZR (phospho T353) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from A-431 cells, untreated or EGF-treated (200 ng/ml, 30 min), using EZR (phospho T353) polyclonal antibody (Cat # PAB18517).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using EZR (phospho T353) polyclonal antibody (Cat # PAB18517).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of EZR.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T353 of human EZR.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to EZR only when phosphorylated at T353.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol).
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-431 cells, untreated or EGF-treated (200 ng/ml, 30 min), using EZR (phospho T353) polyclonal antibody (Cat # PAB18517).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using EZR (phospho T353) polyclonal antibody (Cat # PAB18517).
Peptide "+" means "peptide blocking". -
Gene Info — EZR
Entrez GeneID
7430Protein Accession#
P15311Gene Name
EZR
Gene Alias
CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, VIL2
Gene Description
ezrin
Omim ID
123900Gene Ontology
HyperlinkGene Summary
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017506|OTTHUMP00000017507|cytovillin 2|villin 2 (ezrin)
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Interactome
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Pathway
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Disease
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Publication Reference
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Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport.
Zhao H, Shiue H, Palkon S, Wang Y, Cullinan P, Burkhardt JK, Musch MW, Chang EB, Turner JR.
PNAS 2004 Jun; 101(25):9485.
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Activation of SRC tyrosine kinases in response to ICAM-1 ligation in pulmonary microvascular endothelial cells.
Wang Q, Pfeiffer GR 2nd, Gaarde WA.
Journal of Biological Chemistry 2003 Nov; 278(48):47731.
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Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport.
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