EZR (phospho Thr567/Thr564/Thr558) recombinant monoclonal antibody, clone R03-1G3
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More Files
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Western Blot
Western blot analysis of Lane1: Hela, Lane2: CHO-K1 and Lane3: C6 lysates with EZR (phospho Thr567/Thr564/Thr558) recombinant monoclonal antibody, clone R03-1G3 (Cat # RAB01787 ).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EZR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human EZR.
Theoretical MW (kDa)
Calculated MW: 69 kD
Reactivity
Hamster, Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1/100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1: Hela, Lane2: CHO-K1 and Lane3: C6 lysates with EZR (phospho Thr567/Thr564/Thr558) recombinant monoclonal antibody, clone R03-1G3 (Cat # RAB01787 ).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
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Gene Info — EZR
Entrez GeneID
7430Protein Accession#
P15311Gene Name
EZR
Gene Alias
CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, VIL2
Gene Description
ezrin
Omim ID
123900Gene Ontology
HyperlinkGene Summary
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017506|OTTHUMP00000017507|cytovillin 2|villin 2 (ezrin)
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Interactome
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Pathway
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Disease
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