EZR recombinant monoclonal antibody, clone SAIC-37C-23
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of Hela cells with EZR recombinant monoclonal antibody, clone SAIC-37C-23 (Cat # RAB03434)
Immunofluorescence analysis of paraformaldehyde fixed Hela cells stained with the chimeric r version of RAB03434 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03434
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of Hela cells with EZR recombinant monoclonal antibody, clone SAIC-37C-23 (Cat # RAB03434).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03434 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EZR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against the KLH-conjugated peptide "SGYLSSER" derived from Ezrin.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
Immuno-MRM (multiple reaction monitoring)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of Hela cells with EZR recombinant monoclonal antibody, clone SAIC-37C-23 (Cat # RAB03434)
Immunofluorescence analysis of paraformaldehyde fixed Hela cells stained with the chimeric r version of RAB03434 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03434
(B) DAPI
(C) Merged channels
(D) Isotype controlFlow Cytometry
Flow cytometric analysis of Hela cells with EZR recombinant monoclonal antibody, clone SAIC-37C-23 (Cat # RAB03434).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03434 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Immuno-MRM (multiple reaction monitoring)
-
Gene Info — EZR
Entrez GeneID
7430Gene Name
EZR
Gene Alias
CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, VIL2
Gene Description
ezrin
Omim ID
123900Gene Ontology
HyperlinkGene Summary
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017506|OTTHUMP00000017507|cytovillin 2|villin 2 (ezrin)
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com