CDC25B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from Raw 264.7 cells, treated with UV (15 mins), using CDC25B polyclonal antibody (Cat # PAB18322).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using CDC25B polyclonal antibody (Cat # PAB18322).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using CDC25B polyclonal antibody (Cat # PAB18322).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDC25B.
Immunogen
A synthetic peptide corresponding to residues surrounding S353 of human CDC25B.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to CDC25B.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Raw 264.7 cells, treated with UV (15 mins), using CDC25B polyclonal antibody (Cat # PAB18322).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using CDC25B polyclonal antibody (Cat # PAB18322).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using CDC25B polyclonal antibody (Cat # PAB18322).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC25B
Entrez GeneID
994Protein Accession#
P30305Gene Name
CDC25B
Gene Alias
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Gene Description
cell division cycle 25 homolog B (S. pombe)
Omim ID
116949Gene Ontology
HyperlinkGene Summary
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq
Other Designations
OTTHUMP00000030137|OTTHUMP00000030138|OTTHUMP00000030139|cell division cycle 25B
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Interactome
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Pathway
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Disease
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Publication Reference
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Cdc25B cooperates with Cdc25A to induce mitosis but has a unique role in activating cyclin B1-Cdk1 at the centrosome.
Lindqvist A, Kallstrom H, Lundgren A, Barsoum E, Rosenthal CK.
Journal of Cell Biology 2005 Oct; 171(1):35.
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Binding of 14-3-3beta but not 14-3-3sigma controls the cytoplasmic localization of CDC25B: binding site preferences of 14-3-3 subtypes and the subcellular localization of CDC25B.
Uchida S, Kuma A, Ohtsubo M, Shimura M, Hirata M, Nakagama H, Matsunaga T, Ishizaka Y, Yamashita K.
Journal of Cell Science 2004 Jun; 117(Pt 14):3011.
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Cdc25B cooperates with Cdc25A to induce mitosis but has a unique role in activating cyclin B1-Cdk1 at the centrosome.
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