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Last updated: 2017/6/25
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CDC25B polyclonal antibody

  • Catalog # : PAB18322
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CDC25B.
  • Immunogen:
  • A synthetic peptide corresponding to residues surrounding S353 of human CDC25B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is specific to CDC25B.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    Immunofluorescence (1:500-1:1000)
    ELISA (1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from Raw 264.7 cells, treated with UV (15 mins), using CDC25B polyclonal antibody (Cat # PAB18322).
    Peptide "+" means "peptide blocking".
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using CDC25B polyclonal antibody (Cat # PAB18322).
    Peptide "+" means "peptide blocking".
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells, using CDC25B polyclonal antibody (Cat # PAB18322).
    Peptide "+" means "peptide blocking".
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 994
  • Gene Name:
  • CDC25B
  • Gene Alias:
  • -
  • Gene Description:
  • cell division cycle 25 homolog B (S. pombe)
  • Gene Summary:
  • CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000030137,OTTHUMP00000030138,OTTHUMP00000030139,cell division cycle 25B
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