CDC25B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CDC25B polyclonal antibody (Cat # PAB27029) in extracts from RAW264.7 cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDC25B.
Immunogen
A synthetic peptide corresponding to human CDC25B.
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Human, Mouse, Rat
Specificity
CDC25B polyclonal antibody detects endogenous levels of CDC25B protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDC25B polyclonal antibody (Cat # PAB27029) in extracts from RAW264.7 cells.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC25B
Entrez GeneID
994Protein Accession#
P30305Gene Name
CDC25B
Gene Alias
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Gene Description
cell division cycle 25 homolog B (S. pombe)
Omim ID
116949Gene Ontology
HyperlinkGene Summary
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq
Other Designations
OTTHUMP00000030137|OTTHUMP00000030138|OTTHUMP00000030139|cell division cycle 25B
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Interactome
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Pathway
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Disease
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