SYK polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SYK.
Immunogen
A synthetic peptide corresponding to human SYK.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to SYK.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:300-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells treated with UV (15 mins), using SYK polyclonal antibody (Cat # PAB18139).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SYK polyclonal antibody (Cat # PAB18139).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — SYK
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Interactome
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Pathway
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Disease
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Publication Reference
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The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migration.
Schymeinsky J, Sindrilaru A, Frommhold D, Sperandio M, Gerstl R, Then C, Mocsai A, Scharffetter-Kochanek K, Walzog B.
Blood 2006 Dec; 108(12):3919.
Application:IF, WB, Human, HL-60 cells.
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A cyclic adenosine 3',5'-monophosphate stimulates phospholipase Cgamma1-calcium signaling via the activation of tyrosine kinase in boar spermatozoa.
Harayama H, Murase T, Miyake M.
Journal of Andrology 2005 Nov; 26(6):732.
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Structure and function of Syk protein-tyrosine kinase.
Sada K, Takano T, Yanagi S, Yamamura H.
Journal of Biochemistry 2001 Aug; 130(2):177.
Application:IHC, WB, Human, Cancers, Mammalian cells.
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The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migration.
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