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Last updated: 2017/12/17
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ESPL1 polyclonal antibody

  • Catalog # : PAB18135
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ESPL1.
  • Immunogen:
  • A synthetic peptide corresponding to human ESPL1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is specific to ESPL1.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    Immunofluorescence (1:500-1:1000)
    ELISA (1:40000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from 293 cells, treated with EGF (200 ng/mL, 30 mins), using ESPL1 polyclonal antibody (Cat # PAB18135).
    Peptide "+" means "peptide blocking".
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using ESPL1 polyclonal antibody (Cat # PAB18135).
    Peptide "+" means "peptide blocking".
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HUVEC cells, using ESPL1 polyclonal antibody (Cat # PAB18135).
    Peptide "+" means "peptide blocking".
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9700
  • Gene Name:
  • ESPL1
  • Gene Alias:
  • ESP1,FLJ46492,KIAA0165,SEPARASE,SEPARIN
  • Gene Description:
  • extra spindle pole bodies homolog 1 (S. cerevisiae)
  • Gene Summary:
  • Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM
  • Other Designations:
  • extra spindle poles like 1,separin, separase
  • Gene Pathway
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