ESPL1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with EGF (200 ng/mL, 30 mins), using ESPL1 polyclonal antibody (Cat # PAB18135).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using ESPL1 polyclonal antibody (Cat # PAB18135).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using ESPL1 polyclonal antibody (Cat # PAB18135).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ESPL1.
Immunogen
A synthetic peptide corresponding to human ESPL1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to ESPL1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with EGF (200 ng/mL, 30 mins), using ESPL1 polyclonal antibody (Cat # PAB18135).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using ESPL1 polyclonal antibody (Cat # PAB18135).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using ESPL1 polyclonal antibody (Cat # PAB18135).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ESPL1
Entrez GeneID
9700Protein Accession#
Q14674Gene Name
ESPL1
Gene Alias
ESP1, FLJ46492, KIAA0165, SEPARASE, SEPARIN
Gene Description
extra spindle pole bodies homolog 1 (S. cerevisiae)
Omim ID
604143Gene Ontology
HyperlinkGene Summary
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM
Other Designations
extra spindle poles like 1|separin, separase
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Interactome
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Pathway
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Disease
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Publication Reference
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Regulation of human separase by securin binding and autocleavage.
Waizenegger I, Gimenez-Abian JF, Wernic D, Peters JM.
Current Biology 2002 Aug; 12(16):1368.
Application：IP, WB-Tr, Human, HeLa cells.
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Regulation of human separase by securin binding and autocleavage.
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