ESPL1 (phospho S1126) polyclonal antibody
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More Files
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Immunofluorescence
Immunofluorescent staining of U-2 OS cells with ESPL1 (phospho S1126) polyclonal antibody (Cat # PAB29597) at 1:20-1:100 dilution. Blue: DAPI for nuclear staining.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human ESPL1.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S1126 of human ESPL1.
Host
Rabbit
Theoretical MW (kDa)
233
Reactivity
Human
Specificity
ESPL1 (phospho S1126) polyclonal antibody detects endogenous levels of human ESPL1 only when phosphorylated at serine 1126.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunofluorescence (1:20-1:100)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of U-2 OS cells with ESPL1 (phospho S1126) polyclonal antibody (Cat # PAB29597) at 1:20-1:100 dilution. Blue: DAPI for nuclear staining. -
Gene Info — ESPL1
Entrez GeneID
9700Protein Accession#
Q14674Gene Name
ESPL1
Gene Alias
ESP1, FLJ46492, KIAA0165, SEPARASE, SEPARIN
Gene Description
extra spindle pole bodies homolog 1 (S. cerevisiae)
Omim ID
604143Gene Ontology
HyperlinkGene Summary
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM
Other Designations
extra spindle poles like 1|separin, separase
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Interactome
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Pathway
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Disease
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