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Last updated: 2017/12/10
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DNMT3A polyclonal antibody

  • Catalog # : PAB14129
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of DNMT3A.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 44-58 of human DNMT3A.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100)
    Western Blot (1:2000)
    Immunoprecipitation (2 ug)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4-tagged DNMT3A.
    Whole cell extracts were immunoprecipitated with 2 ug of DNMT3A polyclonal antibody (Cat # PAB14129).
    The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (Lane 1 and 2 respectively).
    Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A polyclonal antibody (Cat # PAB14129) (diluted 1 : 2,000) (Lane 3).
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of DNMT3A polyclonal antibody (Cat # PAB14129), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 500.
  • Application Image
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1788
  • Gene Name:
  • DNMT3A
  • Gene Alias:
  • DNMT3A2,M.HsaIIIA
  • Gene Description:
  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • Gene Summary:
  • CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • DNA MTase HsaIIIA,DNA cytosine methyltransferase 3 alpha,DNA cytosine methyltransferase 3A2,DNA methyltransferase HsaIIIA,OTTHUMP00000120005,OTTHUMP00000120006
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