DNMT3A recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1:Hela whole cell lysate Lane2:The brain tissue lysate of Human Lane3:The heart tissue lysate of Human with DNMT3A recombinant monoclonal antibody (Cat # RAB02755) at 1:1000 dilution.
Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using DNMT3A recombinant monoclonal antibody (Cat # RAB02755)(green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against DNMT3A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant DNMT3A.
Theoretical MW (kDa)
102
Reactivity
Human, Rat
Specificity
This antibody detects endogenous levels of DNMT3A and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:Hela whole cell lysate Lane2:The brain tissue lysate of Human Lane3:The heart tissue lysate of Human with DNMT3A recombinant monoclonal antibody (Cat # RAB02755) at 1:1000 dilution.Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using DNMT3A recombinant monoclonal antibody (Cat # RAB02755)(green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — DNMT3A
Entrez GeneID
1788Protein Accession#
Q9Y6K1Gene Name
DNMT3A
Gene Alias
DNMT3A2, M.HsaIIIA
Gene Description
DNA (cytosine-5-)-methyltransferase 3 alpha
Omim ID
602769Gene Ontology
HyperlinkGene Summary
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
DNA MTase HsaIIIA|DNA cytosine methyltransferase 3 alpha|DNA cytosine methyltransferase 3A2|DNA methyltransferase HsaIIIA|OTTHUMP00000120005|OTTHUMP00000120006
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Interactome
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Pathway
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Disease
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