DNMT3A monoclonal antibody, clone 4G5F11
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DNMT3A-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with DNMT3A monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DNMT3A monoclonal antibody, clone 4G5F11.
Flow Cytometry
Flow cytometric analysis of HeLa cells with DNMT3A monoclonal antibody (green) and negative control (red).
-
Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DNMT3A.
Immunogen
Recombinant protein corresponding to amino acid 46-180 of human DNMT3A from E. coli.
Host
Mouse
Theoretical MW (kDa)
102kDa
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DNMT3A-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with DNMT3A monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DNMT3A monoclonal antibody, clone 4G5F11.Flow Cytometry
Flow cytometric analysis of HeLa cells with DNMT3A monoclonal antibody (green) and negative control (red). -
Gene Info — DNMT3A
Entrez GeneID
1788Gene Name
DNMT3A
Gene Alias
DNMT3A2, M.HsaIIIA
Gene Description
DNA (cytosine-5-)-methyltransferase 3 alpha
Omim ID
602769Gene Ontology
HyperlinkGene Summary
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
DNA MTase HsaIIIA|DNA cytosine methyltransferase 3 alpha|DNA cytosine methyltransferase 3A2|DNA methyltransferase HsaIIIA|OTTHUMP00000120005|OTTHUMP00000120006
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com