RPS6KB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The cell lysate derived from NIH/3T3 cells was immunoprobed by RPS6KB1 polyclonal antibody (Cat # PAB12720) at 1 : 500.
An immunoreactive band is observed around ~70kDa (Lane 1).
This band is blocked by pre-incubation with immunizing peptide (Lane 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RPS6KB1.
Immunogen
A synthetic peptide corresponding to C-terminus of human RPS6KB1.
Host
Rabbit
Theoretical MW (kDa)
70
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~70 KDa of human RPS6KB1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from NIH/3T3 cells was immunoprobed by RPS6KB1 polyclonal antibody (Cat # PAB12720) at 1 : 500.
An immunoreactive band is observed around ~70kDa (Lane 1).
This band is blocked by pre-incubation with immunizing peptide (Lane 2).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS6KB1
Entrez GeneID
6198Gene Name
RPS6KB1
Gene Alias
PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha
Gene Description
ribosomal protein S6 kinase, 70kDa, polypeptide 1
Omim ID
608938Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
p70 S6 kinase, alpha 1|p70 S6 kinase, alpha 2|ribosomal protein S6 kinase, 70kD, polypeptide 1|serine/threonine kinase 14 alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Signaling from Akt to FRAP/TOR targets both 4E-BP and S6K in Drosophila melanogaster.
Miron M, Lasko P, Sonenberg N.
Molecular and Cellular Biology 2003 Dec; 23(24):9117.
Application：WB-Tr, Drosophila, S2 cells.
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Signaling from Akt to FRAP/TOR targets both 4E-BP and S6K in Drosophila melanogaster.
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