RPS6KB1 (phospho T421/S424) recombinant monoclonal antibody, clone 3B6
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More Files
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Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 50 nM/60 mins) and Lane 2: 293 whole cell lysate (not treated) with RPS6KB1 (phospho T421/S424) recombinant monoclonal antibody, clone 3B6 (Cat # RAB04230).
Immunofluorescence
Immunofluorescent staining of Hela cells with RPS6KB1 (phospho T421/S424) recombinant monoclonal antibody, clone 3B6 (Cat # RAB04230) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with RPS6KB1 (phospho T421/S424) recombinant monoclonal antibody, clone 3B6 (Cat # RAB04230).
Lane 1: rabbit control IgG, Lane 2: RAB04230 precipitates and Lane 3 : Input (Hela whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RPS6KB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding T421/S424 of human RPS6KB1.
Theoretical MW (kDa)
Calculated MW: 70 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 50 nM/60 mins) and Lane 2: 293 whole cell lysate (not treated) with RPS6KB1 (phospho T421/S424) recombinant monoclonal antibody, clone 3B6 (Cat # RAB04230).Immunofluorescence
Immunofluorescent staining of Hela cells with RPS6KB1 (phospho T421/S424) recombinant monoclonal antibody, clone 3B6 (Cat # RAB04230) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with RPS6KB1 (phospho T421/S424) recombinant monoclonal antibody, clone 3B6 (Cat # RAB04230).
Lane 1: rabbit control IgG, Lane 2: RAB04230 precipitates and Lane 3 : Input (Hela whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS6KB1
Entrez GeneID
6198Protein Accession#
P23443Gene Name
RPS6KB1
Gene Alias
PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha
Gene Description
ribosomal protein S6 kinase, 70kDa, polypeptide 1
Omim ID
608938Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
p70 S6 kinase, alpha 1|p70 S6 kinase, alpha 2|ribosomal protein S6 kinase, 70kD, polypeptide 1|serine/threonine kinase 14 alpha
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Interactome
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Pathway
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Disease
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