MAP2K1/MAP2K2 (phospho S218/S222) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The whole cell lysates derived from Starved NIH/3T3 were immunoblotted by MAP2K1/MAP2K2 (phospho S218/222) polyclonal antibody (Cat # PAB12635) at 1 : 1000 (lane 1). The lane 2 was a negative control.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : Phospho-MAP2K1/MAP2K2 (pS218/222).
B : MAP2K1/MAP2K2 (Nonphospho).
C : Non-related phospho-specific phosphopeptide.
Followed were blotted at a 1 : 1000 dilution by :
1. MAP2K1/MAP2K2 (phospho S218/222) polyclonal antibody (Cat # PAB12635).
2. MAP2K1/MAP2K2 polyclonal antibody (Cat # PAB12707).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1/MAP2K2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S218/S222 of human MAP2K1/MAP2K2.
Sequence
SMANS
Host
Rabbit
Theoretical MW (kDa)
44, 45
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~44/45 KDa of human MAP2K1/MAP2K2 at the phosphorylation site of Ser 218/222 or MEK2(pS222/226). It does not cross react to non-phospho MEK1/2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysates derived from Starved NIH/3T3 were immunoblotted by MAP2K1/MAP2K2 (phospho S218/222) polyclonal antibody (Cat # PAB12635) at 1 : 1000 (lane 1). The lane 2 was a negative control.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : Phospho-MAP2K1/MAP2K2 (pS218/222).
B : MAP2K1/MAP2K2 (Nonphospho).
C : Non-related phospho-specific phosphopeptide.
Followed were blotted at a 1 : 1000 dilution by :
1. MAP2K1/MAP2K2 (phospho S218/222) polyclonal antibody (Cat # PAB12635).
2. MAP2K1/MAP2K2 polyclonal antibody (Cat # PAB12707). -
Gene Info — MAP2K1
Entrez GeneID
5604Gene Name
MAP2K1
Gene Alias
MAPKK1, MEK1, MKK1, PRKMK1
Gene Description
mitogen-activated protein kinase kinase 1
Omim ID
176872Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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Gene Info — MAP2K2
Entrez GeneID
5605Gene Name
MAP2K2
Gene Alias
FLJ26075, MAPKK2, MEK2, MKK2, PRKMK2
Gene Description
mitogen-activated protein kinase kinase 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq
Other Designations
ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|dual specificity mitogen-activated protein kinase kinase 2|mitogen-activated protein kinase kinase 2, p45
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Interactome
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Pathway
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Disease
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Publication Reference
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Inhibition of the Raf-MEK1/2-ERK1/2 signaling pathway, Bcl-xL down-regulation, and chemosensitization of non-Hodgkin's lymphoma B cells by Rituximab.
Jazirehi AR, Vega MI, Chatterjee D, Goodglick L, Bonavida B.
Cancer Research 2004 Oct; 64(19):7117.
Application：WB-Ce, Human, Ramos, Daudi cells.
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Inhibition of the Raf-MEK1/2-ERK1/2 signaling pathway, Bcl-xL down-regulation, and chemosensitization of non-Hodgkin's lymphoma B cells by Rituximab.
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