MAP2K1 (phospho S218/S222) recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:A431 whole cell lysate Lane2:Hela whole cell lysate Lane3:293T whole cell lysate with MAP2K1 (phospho S218/S222) recombinant monoclonal antibody (Cat # RAB02767) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using MAP2K1 (phospho S218/S222) recombinant monoclonal antibody (Cat # RAB02767). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HepG2 cells using MAP2K1 (phospho S218/S222) recombinant monoclonal antibody (Cat # RAB02767)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against MAP2K1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant MAP2K1.
Theoretical MW (kDa)
43
Reactivity
Human
Specificity
This antibody detects endogenous levels of MEK1 protein only when phosphorylated at Ser218 and/or Ser222.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:A431 whole cell lysate Lane2:Hela whole cell lysate Lane3:293T whole cell lysate with MAP2K1 (phospho S218/S222) recombinant monoclonal antibody (Cat # RAB02767) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using MAP2K1 (phospho S218/S222) recombinant monoclonal antibody (Cat # RAB02767). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HepG2 cells using MAP2K1 (phospho S218/S222) recombinant monoclonal antibody (Cat # RAB02767)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — MAP2K1
Entrez GeneID
5604Protein Accession#
Q02750Gene Name
MAP2K1
Gene Alias
MAPKK1, MEK1, MKK1, PRKMK1
Gene Description
mitogen-activated protein kinase kinase 1
Omim ID
176872Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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Interactome
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Pathway
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Disease
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