MAP2K2 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against MAP2K2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant MAP2K2.
Theoretical MW (kDa)
43
Reactivity
Human, Mouse, Rat, Zebra fish
Specificity
This antibody detects endogenous levels of MEK1/2 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:HepG2 whole cell lysate Lane2:PC12 whole cell lysate Lane3:NIH/3T3 whole cell lysate with MAP2K2 recombinant monoclonal antibody (Cat # RAB02698) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using MAP2K2 recombinant monoclonal antibody (Cat # RAB02698). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of A549 cells using MAP2K2 recombinant monoclonal antibody (Cat # RAB02698)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — MAP2K2
Entrez GeneID
5605Protein Accession#
P36507;Q02750Gene Name
MAP2K2
Gene Alias
FLJ26075, MAPKK2, MEK2, MKK2, PRKMK2
Gene Description
mitogen-activated protein kinase kinase 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq
Other Designations
ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|dual specificity mitogen-activated protein kinase kinase 2|mitogen-activated protein kinase kinase 2, p45
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