MAP2K1/MAP2K2 recombinant monoclonal antibody, clone R08-6A7
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with MAP2K1/MAP2K2 recombinant monoclonal antibody, clone R08-6A7 (Cat # RAB02109).
Immunocytochemistry
Immunocytochemical staining of Hela with MAP2K1/MAP2K2 recombinant monoclonal antibody, clone R08-6A7 (Cat # RAB02109). (A) MAP2K1/MAP2K2 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MAP2K1/MAP2K2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human MAP2K1/MAP2K2.
Theoretical MW (kDa)
Calculated MW: 43,44
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with MAP2K1/MAP2K2 recombinant monoclonal antibody, clone R08-6A7 (Cat # RAB02109).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of Hela with MAP2K1/MAP2K2 recombinant monoclonal antibody, clone R08-6A7 (Cat # RAB02109). (A) MAP2K1/MAP2K2 (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — MAP2K1
Entrez GeneID
5604Protein Accession#
Q02750;P36507Gene Name
MAP2K1
Gene Alias
MAPKK1, MEK1, MKK1, PRKMK1
Gene Description
mitogen-activated protein kinase kinase 1
Omim ID
176872Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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Gene Info — MAP2K2
Entrez GeneID
5605Protein Accession#
Q02750;P36507Gene Name
MAP2K2
Gene Alias
FLJ26075, MAPKK2, MEK2, MKK2, PRKMK2
Gene Description
mitogen-activated protein kinase kinase 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq
Other Designations
ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|dual specificity mitogen-activated protein kinase kinase 2|mitogen-activated protein kinase kinase 2, p45
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Interactome
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Pathway
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Disease
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